U251-Luc
CBP30207L
| I. Introduction | |
| Host Cell: | U251 |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM + 1% Non Essential Amino Acids NEAA + 1mM Sodium Pyruvate NaP + 10% FBS + 2μg/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Brain |
| Disease: | Human glioblastoma astrocytoma |
| Morphology: | Pleomorphic/astrocytoid |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). The number of Luminescence signal is 508±6(Mean±SD) on U251 cells while 49992742 ±1810197(Mean±SD) on U251-Luc cells. |
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| IV. Stablity Test | |
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Figure 2. Stability test results. U251-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
BALB/c Nude；NOD/SCID |
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Implanted Position |
脑原位 |
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Injected Cell Numbers |
1×10^5/4μL |
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Matrix gel |
Without matrix gel |
BALB/c Nude
1. Weight Change
Figure 3. U251-LUC-BALB/c Nude Orthotopic Tumor Model.
Figure 4. U251-LUC-BALB/c Nude Orthotopic Tumor Model.
2. Tumor Flux
Figure 5. U251-LUC-BALB/c Nude Orthotopic Tumor Model.
NOD/SCID
1. Weight Change
Figure 6. U251-LUC-NOD/SCID Orthotopic Tumor Model.
Figure 7. U251-LUC-NOD/SCID Orthotopic Tumor Model.
2. Tumor Flux
Figure 8. U251-LUC-NOD/SCID Orthotopic Tumor Model.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
